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Thursday, July 30, 2020 | History

4 edition of DNA polymerase activity in trophoblast giant cells in the mouse found in the catalog.

DNA polymerase activity in trophoblast giant cells in the mouse

by Wendy L. Dean

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  • 1 Currently reading

Published by Dept. of Biological Sciences, Brock University in St. Catharines [Ont.] .
Written in English

    Subjects:
  • DNA polymerases.,
  • Trophoblast.,
  • Mice -- Physiology.,
  • Cells.

  • Edition Notes

    Statementby W.L. Dean. --
    The Physical Object
    Paginationviii, 155 leaves :
    Number of Pages155
    ID Numbers
    Open LibraryOL23357895M

      DNA molecules are the troves of genetic information of an organism. DNA is the basis of life and is transferred from parent to offspring's. The DNA content of the parent is doubled by means of replication mechanism aided by a specific enzyme, DNA polymerases. DNA polymerase plays a central role in process of life and carries a weighty responsibility of making an accurate copy of the cell's . Geminin prevents trophoblast stem cells from exiting mitosis and differentiating into the nonproliferating, viable, giant cells essential for implantation and placentation. The protein geminin is involved in both DNA replication and cell fate acquisition. Although it is essential for mammalian preimplantation development, its role remains unclear.

    activity in transfected Rcho-1 cells (Shida et al., ; Ng et al., ), a rat choriocarcinoma cell line that differentiates in culture into hormone-producing giant cells (Faria and Soares, ). Furthermore, we have demonstrated that GATA-2 and GATA-3 are expressed in trophoblast giant cells.   Transient Transfection and luciferase activity. Human trophoblast cell line HTR-8/SVneo (HTR) and non-trophoblast cell lines HEK (human embryonic kidney), HeLa (human cervical carcinoma), M1 (renal tubular epithelial), A (human lung epithelial), ML12 (mouse lung epithelial)and K (human leukemia)were plated on 6-well plates at ×

      Here, we show that Hand1 gene, which codes for a transcription factor crucial for differentiation of trophoblast giant cells and heart development, is upregulated in hmga1 minus embryonic stem cells. Trophoblast stem (TS) cells then differentiate into the polyploid, viable, nonproliferating trophoblast giant (TG) cells required for embryo implantation and placentation (Figure 5). PreRC assembly requires the absence of both CDK activity and geminin, a condition that occurs in mitotic cell cycles during the anaphase-to-G1 phase transition.


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DNA polymerase activity in trophoblast giant cells in the mouse by Wendy L. Dean Download PDF EPUB FB2

Inordertodeterminewhethera'switch'fromDNApolymerase toDNA polymerase isa necessaryconcomitant ofthe diploid to giant cell transformation, two distinctpopulations of trophoblastgiant cells,the primary.

These results suggest that although DNA polymerase a is the primary replicating enzyme responsible for endoreduplication in mouse trophoblast giant cells, some nonactivity is also observed. A DNA polymerase assay employing tissue lysates of outgrown da embryo, EX and EPC tissues was used to attempt to confirm the presence of higher Author: Wendy L.

Dean. The cells are not of the same cell lineage as trophoblast giant cells in the rat. Since we were unable to detect immunoreactive signals by antibodies against rat KiSS-1 proteins, probably because of their low efficiency, it is still unclear whether the molecule would also express in syncytiotrophoblast cells in rat by: Trophoblast progenitor cells exit the mitotic cell cycle and undergo rounds of DNA replication without intervening mitoses (endoreduplication), leading to increased ploidy.

High-resolution quantification of DNA content shows that small regions of the genome are relatively under-replicated (∼5% of genome in 47 loci) and others over-replicated Cited by: 1.

Ensembl ENSG ENSMUSG UniProt O Q RefSeq (mRNA) NM_ NM_ RefSeq (protein) NP_ NP_ Location (UCSC) Chr 5: – Mb Chr – Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Heart- and neural crest derivatives-expressed protein 1 is a protein that in humans is encoded by the HAND1 Aliases: HAND1, Hxt, Thing1, bHLHa27, eHand.

mechanism of giant cell DNA amplification involves multiple rounds of DNA replication in the absence of both karyokinesis and cytokinesis, and that sister chromatids, but not homologous chromosomes, re-main closely associated during this process.

Key words: mouse, trophoblast, giant cells, polytene chromosome, DNA replication. Introduction. cell lines to somatic cell types, which could also be applied for genetic screening (He et al., ; Sagi et al., ).

However, no haploid cell line derived from the trophoblast lineage has yet been reported. Here, we report the successful establishment of mouse parthenogenetic haploid TS cells. In line with our study, differentiation of mouse trophoblast stem cells into (predominantly) trophoblast giant cells is associated with decreased acetylation of H2AK5, H2BK5, H2BK12, H2BK These results indicate that the resumption of DNA synthesis is part of the overall increase in metabolic activity associated with activation.

Furthermore, the sequential pattern of resumption of synthesis suggests that the ICM may influence the initiation of DNA synthesis in the surrounding trophoblast.

A stem cell population of the trophectoderm lineage can be isolated and maintained in vitro under the presence of fibroblast growth factor 4, heparin, and a feeder layer of mouse embryonic fibroblast cells. Such apparently immortal stem cells, trophoblast stem (TS) cells, exhibit the potential to differentiate to multiple cell types in vitro.

Y-box binding protein 1 (YB-1) is pivotal for the regulation of cancerogenesis and inflammation. However, its involvement in pregnancy processes such as fetal and placental development remains to be elucidated. We studied Ybx1 (YB-1)+/− heterozygous intercrossings and compared them to YB-1+/+ wild-type (WT) combinations.

Additionally, we generated trophoblast-specific YBdeficient mice by. Ullah, Z., C. de Renty and M.L. DePamphilis () Propagation And Differentiation Of Mouse Trophoblast Stem Cells", Intramural Pluripotent Stem Cell Protocol Book VersionHunsberger, Joshua, Mahendra Rao, and Steven Becker (eds).

The transcription factor Oct‐4 is a marker of pluripotency in mouse and human embryonic stem (ES) cells. Previous studies using a tetracycline‐regulated Oct‐4 transgene in the ZHBTc4 cell line demonstrated that downregulation of Oct‐4 expression induced dedifferentiation into trophoblast, a lineage mouse ES cells do not normally generate.

entiate into secondary trophoblast giant cells, which lie in the periphery of the placenta, forming the interface with maternal cells in the decidua (1, 2). The expression of placental lactogen (PL) is regulated during trophoblast giant cell differentiation (3).

The gene is specific for a group of cells of the. The mouse is often used as a model for understanding human placentation and offers multiple advantages, including the ability to manipulate gene expression in specific compartments and to derive trophoblast stem cells, which can be maintained or differentiated in heless, there are numerous differences between the mouse and human placentas, only the least of which are structural.

Trophoblasts are the first cell type to be specified during embryogenesis, and they are essential for placental morphogenesis and function. Trophoblast stem (TS) cells are the progenitor cells for all trophoblast lineages; control of TS cell differentiation into distinct trophoblast subtypes is not well understood.

Mice lacking the transcription factor OVO-like 2 (OVOL2) fail to produce a. George F. Kalf's 73 research works with 1, citations and reads, including: The Interaction of 1,4-Benzoquinone, a Bioreactive Intermediate of Benzene, with Three Proteins Essential for. Fusion of trophoblast giant cells with secondary oocytes, which are rich in maturation promoting factor (MPF) activity, revealed that an exogenous source of active MPF could cause chromosome.

Mice homozygous for a knock-out allele exhibit embryonic lethality between E and E due to failure of proliferation of the inner cell mass and trophoblast giant cells.

Mice heterozygous for this allele exhibit age-related decreased male germ cells due to impaired meiosis, shortened telomeres and chromosomal instability. A DNA polymerase is a member of a family of enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, the molecular precursors of enzymes are essential for DNA replication and usually work in groups to create two identical DNA duplexes from a single original DNA duplex.

During this process, DNA polymerase "reads" the existing DNA strands to create two new. Periodic expression of the cyclin-dependent kinase inhibitor p57(Kip2) in trophoblast giant cells defines a G2-like gap phase of the endocycle.

Molecular biology of the cell. ; – [PMC free article] Himeno E, Tanaka S, Kunath T. Isolation and manipulation of mouse trophoblast stem cells.The trophoblast can be modeled in vitro using trophoblast stem cells. Trophoblast stem cells require fibroblast growth factor (FGF)4, heparin, and contact with .Article The Transcription Factor OVOL2 Represses ID2 and Drives Differentiation of Trophoblast Stem Cells and Placental Development in Mice Mariyan J.

Jeyarajah 1, Gargi Jaju Bhattad 1, Dendra M. Hillier 1 and Stephen J. Renaud 1,2,3,* 1 Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A5C1, Canada; .